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  • Methods and Protocols.
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Moreover, GFP is detectable directly in living cells, whereas GUS is only detected indirectly by staining of fixed tissue. This may lead to artifacts or it may obscure problems with protein solubility. In this protocol, protein localization is routinely assayed after particle-mediated transient transformation of onion epidermal cells. With this method it can be determined rapidly whether a given fusion protein is active, and preliminary targeting data can be obtained. Terms of Service.

Establishment of 3D organotypic cultures using human neonatal epidermal cells.

Full Text. Russell Cold Spring Harb Protoc ; ; doi: This Article doi Article Category Protocol. Authors: Hua Qian 1 ,. Xue Leng 1 ,. Jie Wen 1 ,.

Qian Zhou 1. Xunwei Wu 1. Hua Qian 1 ,.

Qian Zhou 1 ,. Xin Xu 1 ,.


  • The Family Law.
  • Subcellular Localization of GUS- and GFP-Tagged Proteins in Onion Epidermal Cells.
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Full text PDF Related articles. Abstract Stem cells play a crucial role in maintaining and repairing tissues during homeostasis and following injury. Related articles Based on techniques.

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Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture

Torre-Healy et al. Judson et al.

Layers of the Epidermis

Atif et al. Dev Cell 43 4 —